BCSnob
Middletown, MD
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CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel
This is from page 31 of the procedure from the cdc on running the real time PCR assay.
Quote:16) Before proceeding, the run file must be saved; from the main menu, select File, then Save As. Save in appropriate run folder designation.
17) Load the plate into the plate holder in the instrument. Ensure that the plate is properly aligned in the holder.
18) Once the run file is saved, click the Start button. Note: The run should take approximately 1 hour and 20 minutes to complete.
The time is due to the thermal cycling required during the amplification process.
This is after quite a lot of prep work getting everything ready.
The fastest rtPCR test results (sample collection to test results) I’ve found listed on-line is 2hrs for a clinic that runs the test onsite laboratory for several hundred $.
Which PCR test (or testing lab) gave results in ~1hr?
Never mind I found Cepheid, Abbott & ThermoFisher have low volume (1 sample at a time) molecular tests (tests for specific pieces of RNA) that provide results in 13-36min.
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Deb and Ed M
SW MI & Space Coast, FL USA
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BCSnob wrote:
Never mind I found Cepheid, Abbott & ThermoFisher have low volume (1 sample at a time) molecular tests (tests for specific pieces of RNA) that provide results in 13-36min.
OK - that made me burst out laughing - people are sitting in lines of cars for many, many hours to get tested in Orlando. One-sample-at-a-time isn't going to be much help right now ;-)
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BCSnob
Middletown, MD
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There’s trade off between fast turnaround time and high throughput (high volume). You’re right 1 at a time isn’t much; at work I’ve run >5,000 samples at a time. It takes a lot of focus to not screw it up or very expensive automation to run that many samples.
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way2roll
Wilmington NC
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Just read an article that agrees with what I have been saying for a while now. omicron is so contagious to unvaccinated and vaccinated alike that it could potentially snuff itself out in herd immunity.
The high-transmission rate of the omicron variant of COVID-19 could potentially mean that as it rips its way through the U.S. population leaving antibodies in its wake, herd immunity may actually be possible.
In a recent CNBC report, Dr. David Ho, a world-renowned virologist, posited that “Sometimes a rapid-fire could burn through very quickly but then put itself out.”
Article
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Deb and Ed M
SW MI & Space Coast, FL USA
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Dr Scott Gottlieb has implied the same fast-burnout scenario, too Clicky
And on edit - this can't happen fast enough. The Covid numbers are insane in FL right now and testing lines are hours-long. Makes me wonder how many people simply aren't getting tested and tough it out at home with rest and Tylenol?
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BCSnob
Middletown, MD
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It looks like in FL & NC the rise in the hospitalization curve for the omicron wave is just as steep as it was for the delta wave. It looks steeper for omicron than delta in MD.
* This post was
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way2roll
Wilmington NC
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BCSnob wrote:It looks like in FL & NC the rise in the hospitalization curve for the omicron wave is just as steep as it was for the delta wave. It looks steeper for omicron than delta in MD.
Do you think it's possible that a lot of the hospitalizations are a results of more people going to the Er out of fear? Is it possible that many of these people would normally ride out these symptoms at home? If Omicron symptoms are much milder than Delta, could hysteria be causing some of the hospitals to be overburdened?
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BCSnob
Middletown, MD
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Short answer:
No
Long answer:
ERs in our area are calling surrounding hospitals to send patients to since most have no beds to admit patients from the ER. If current patients or those going to the ERs don’t need hospitalization the ERs would not be trying to admit taking up a bed. We know someone who went in to UMD Medical Center in Baltimore for a surgery (not Covid related) and once she no longer needed ICU care after surgery they had no beds outside of ICU. She was discharged from ICU to a rehab facility.
Quote:By the end of the day, about 300 more patients had been added to the count, pushing the total above 3,000, said Bob Atlas, president and CEO of the Maryland Hospital Association. That data will not be posted on the state’s coronavirus dashboard until Tuesday morning.
By the association’s count, nine of the state’s 44 acute care hospitals have entered crisis mode, including the University of Maryland St. Joseph Medical Center in Towson and Frederick Health Hospital, which adopted the emergency protocols Monday.
“Maryland hospitals have been sounding the alarm for the past month — noting that the situation would soon become dire. That has happened,” Atlas said in an email, adding that most hospitals had reached full capacity. “This means that any patient who is not in need of immediate, urgent, emergency care, may have to wait until there is staffing and/or a bed available to treat them.”
Maryland hit 3,000 COVID-related hospita........ations Monday, hospital association says
Md. state of emergency helps hospitals overwhelmed by COVID-19 patients
While ERs may have many people seeking care that is less than what requires hospitalization; there are more people in the ERs that do need hospitalization than there are beds in the hospitals.
Keep in mind there are ER beds, ICU beds, and in-patient beds in a hospital; in-patient and ICU beds are used for the hospitalization figures.
Wait, 2 weeks and we’ll revisit your questions; the start of the omicron wave in NC appears about 2 weeks behind those in FL and MD.
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dturm
Lake County, IN
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We have a friend who had to go to the ER not COVID related and waited 24 hrs in the ER for a bed. Our son needs to have an angioplasty, fortunately not an emergency situation and has it scheduled for later in the month.
As Mark said, people may go to ER scared, but they don't get admitted because of scare.
While generally omicron symptoms appear less severe and less lethal, it can still be a serious disease in many individuals (NON-VACCINATED, and those with co-morbidities).
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Deb and Ed M
SW MI & Space Coast, FL USA
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I think here in FL, it might be a case of people deciding to "ride out" whatever illness they are experiencing, because the waiting lines for Covid testing are hours-long. Of course, a certain amount of those choosing to tough it out, get sicker; and then head to the ER. There's good news as of today (I hope!) - the overall new case numbers for FL Covid took a teeeeeny dip. It's still more than double the previous peak for Delta - but hopefully the state has reached a burnout stage... at least until the Spring Breakers start showing up or the next variant-of-concern shows up.
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